Phytochemical Screening of Rhizome Extract of Curcuma longa Linn. Grown in Bangladesh through GC-MS and its Bioactivities

The methanolic extract of Curcuma longa L. rhizome was subjected to Gas Chromatography-Mass Spectrometric (GC-MS) analysis. chromatographic investigation the showed 27 constituents which 19 compounds were newly found from C. longa. Here, peaks obtained in total ion chromatogram compared with National Institute Standards and Technology (NIST) database for identification constituents. most plentiful component 9-octadecenamide (Z) (5.93%). present lowest amount 4-hydroxy-3-methoxy benzoic acid methyl ester (0.08%). its six fractions (F1-F6) through vacuum liquid chromatography (VLC) also investigated antioxidant, cytotoxic antimicrobial activities.The crude (F4-F6) exhibited strong antioxidant activity having IC50 values 19.85, 18.41, 26.72 16.79 μg/ml, respectively standards butylated hydroxyl anisole (IC50 = 11.42 μg/ml) ascorbic 9.01 μg/ml). Strong cytotoxicity (LC50 1.81 F4 2.4 μg/ml), F5 2.5 F6 2.3 when standard, tamoxifen 0.13 Fractions F1-F3 displayed mild moderate antibacterial against Bacillus megaterium, Staphylococcus aureus Pseudomonas aeruginosa comparison Kanamycin. GC-MS may explain above activities. results prospect this medicinal plant explore further determine bioactive components. Dhaka Univ. J. Pharm. Sci. 22(1): 11-20, 2023 (June)